ABSTRACT
Objective To detect the expressions of Matrix Metalloprotein-11 ( MMP-11 ) and Cathepsin-D(Cath-D),and investigate their relationship and prognostic significance. Methods The study included 95 cases′ clinical date and postoperative specimens of gastric adenocarcinoma ( North China University of Science and Technology Affiliated Hospital,2010. 01-2013. 12) as observation group,70 cases of normal gastric tissue(from observation group) as control group. Expressions of MMP-11 and Cath-D were detected by IHC methods in two groups. Results The positive rate of MMP-11 was 51. 6%( 49/95) in observation group,5. 7%(4/70)in control group(χ2＝38. 884,P＜0. 05). The positive rate of Cath-D was 73. 7%(70/95) in observation group,28. 6%(20/70) in control group(χ2＝33. 082,P＜0. 05). The positive rate of MMP-11 was correlated with metastasis and vascular invasion(χ2＝7. 193、15. 566,P＜0. 05). The positive rate of Cath-D was correlated with maximal diameter,lymph node metastasis,vascular invasion and proliferation index(χ2＝7.431、5.654、6.569、6.801,P＜0.05).There was positive relationship between MMP-11 and Cath-D in observation group(r＝0. 46,P＜0. 05). The expressions of MMP-11 and Cath-D were correlated with prognosis in gastric adenocarcinoma ( P＜0. 05) . Conclusion The higher expressions and synergistic effect of MMP-11 and Cath-D may promote the occurrence and development in gastric adenocarcinoma. The joint detection of MMP-11 and Cath-D may be helpful to predict the prognosis of gastric adenocarcinoma.
ABSTRACT
El cáncer de mama (CM) es uno de los más frecuentes en Argentina y primera causa de muerte por cáncer en mujeres. Las metaloproteasas son endopeptidasas que degradan la matriz extracelular, facilitando la invasión tumoral y las metástasis. Se observó la utilidad de la MMP-9 como un marcador diagnóstico, pronóstico y de seguimiento en pacientes con CM. La MMP-11 parece tener un efecto dual en cáncer, su aumento se asocia a un incremento de tumores primarios de mama, pero con una represión en el desarrollo de metástasis. En el trabajo se analizaron los polimorfismos de nucleótido único (SNPs) Arg574Pro del gen MMP-9 y Ala38Val del MMP-11, con relación a las metástasis de CM. Se tomaron muestras de sangre de pacientes con CM metastásico y no metastásico (controles), con receptores de progesterona+, estrógeno+ y HER2-neu+/-. Se extrajo ADN de 25 muestras y se diseñaron cebadores para amplificar la región que contenían los SNPs Arg574Pro y Ala38Val. Se estandarizó la PCR para los SNPs correspondientes, aclarando que el cebador izquierdo que amplifica Arg574Pro, hibrida sobre los polimorfismos rs146961494 y rs35691798. Se realizó el análisis de las enzimas de restricción, MbiI para Arg574Pro y AatII para Ala38Val. Se concluye que para MMP-9, el polimorfismo presenta el alelo C como el G sólo en el grupo metastásico. En cuanto al gen MMP-11, se encuentra en alta frecuencia la variante alélica T, la cual no corresponde al alelo ancestral, indicando que puede estar su función/expresión relacionada con el carcinoma mamario. Estos hallazgos son preliminares (AU)
Breast cancer (BC) is one of the most common in Argentina and the leading cause of cancer death in women. Metalloproteases are endopeptidases that degrade the extracellular matrix, facilitating tumor invasion and metastases. The utility of MMP-9 was observed as a diagnostic, prognostic and follow-up marker in BC patients. MMP-11 appears to have a dual effect on cancer. High levels are associated with an increase in primary breast tumors, but with repression in the development of metastases. Arg574Pro SNPs of the MMP-9 gene and Ala38Val of MMP-11 gene were analyzed in relation to BC metastases. Blood samples were taken from patients with BC metastatic and non-metastatic (controls), with progesterone+, estrogen+ and HER2-neu+/- receptors. DNA from 25 samples was drawn and primers were designed to amplify the region containing the SNPs Arg574Pro and Ala38Val. PCR was standardized for the corresponding SNPs, clarifying that the Arg574Pro amplified left primer hybridizes to polymorphisms rs146961494 and rs35691798. The restriction enzyme analysis was performed, MbiI for Arg574Pro and AatII for Ala38Val. It is concluded that for MMP-9, the polymorphism presents the C allele as the G only in the metastatic group. As for the MMP-11 gene, the allelic variant T is found in high frequency, which does not correspond to the ancestral allele, indicating that its function/expression may be related to mammary carcinoma. These findings are preliminary (AU)
Subject(s)
Humans , Breast Neoplasms , Neoplasm Metastasis , Polymorphism, Single Nucleotide , MetalloproteasesABSTRACT
Objective To investigate the influence of Let-7f regulation on MMP-11 in migration and in-vasion of pancreatic cancer. Methods RT-qPCR was performed to assay the expression of Let7 and MMP-11 ex-pression in pancreatic cancer cells and normal pancreatic cells. Let-7f mimic and inhibitor were transfected to PANC-1 and Bx-PC3, respectively. The influence of Let-7f expression on migration and invasion of pancreatic cancer cells was analyzed by transwell. The influence of Let-7f intervention on MMP-11 expression was observed by RT-qPCR. Results Let-7f expression decreased in PANC-1 and Bx-PC3, compared to HPDE6c7, while MMP-11 expression up-regulated in PANC-1 and Bx-PC3 , compared to HPDE6c7 cells. Let-7f overexpression in-hibited the expression of MMP-11. Up-regulated Let-7f could significantly inhibit migration and invasion of PANC-1 and Bx-PC3 and Let-7f inhibitor enhanced MMP-11 expression. However , down-expression of Let-7f could not significantly promote migration and invasion abilities of PANC-1 and Bx-PC3cells. Conclusions Let-7f may affect migration and invasion by regulating MMP-11 expression in pancreatic cancer , suggesting that Let-7f might be a potential target for gene therapy in human pancreatic cancer.